Composite
Ag43+FLAG-

Part:BBa_K1352002:Design

Designed by: Martyna Sroka, Ana-Maria Cujba   Group: iGEM14_Aberdeen_Scotland   (2014-10-03)


Ag43+FLAG+MCS(-)beta-hairpins under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1678
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 3103
    Illegal NgoMIV site found at 3613
    Illegal NgoMIV site found at 3634
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2629
    Illegal AgeI site found at 3373
    Illegal AgeI site found at 3787
    Illegal AgeI site found at 4029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Maintaining ORF integrity


Source

PCR amplified from Bba_K1352001; two hairpin sequences (H1 268 AATVTGTNRLGAFSVVA 284; H2 341 GAAVSGTRSDGKAFSIG 357) were removed via In-Fusion (Clonetech).


References